Download e-book for iPad: An introduction to genetic engineering by Dr Desmond S. T. Nicholl

By Dr Desmond S. T. Nicholl

ISBN-10: 0521615216

ISBN-13: 9780521615211

During this 3rd variation of his well known undergraduate-level textbook, Des Nicholl recognises sound seize of easy rules is key in any advent to genetic engineering. as a result, in addition to being completely up-to-date, the e-book additionally keeps its specialise in the elemental ideas utilized in gene manipulation. The textual content is split into 3 sections: half I presents an creation to the proper easy molecular biology; half II, the tools used to control genes; and half III, purposes of the expertise. there's a new bankruptcy dedicated to the rising value of bioinformatics as a special self-discipline. different extra gains comprise textual content containers, which spotlight very important facets of issues mentioned, and bankruptcy summaries, which come with goals and studying results. those, in addition to key observe listings, thought maps and a thesaurus, will let scholars to tailor their examine to fit their very own studying kinds and eventually achieve a company take hold of of an issue that scholars typically locate tough.

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Viral and bacterial genomes tend to show very efficient use of DNA for encoding their genes, which is a consequence of (and explanation for) their small genome size. However, in the human genome, only about 3% of the total amount of DNA is actually coding sequence. Even when the introns and control sequences are added, the majority of the DNA has no obvious function. This is sometimes termed ‘junk’ DNA, although this is perhaps the wrong way to think about this apparently redundant DNA. Estimating the number of genes in a particular organism is not an exact science, and a number of different methods may be used.

The difference between labelling for tracing purposes and labelling for probes is largely one of specific activity, that is, the measure of how radioactive the molecule is. For tracing purposes, a low specific activity will suffice, but for probes a high specific activity is necessary. In probe preparation the radioactive label is usually the high-energy ß-emitter 32 P. Some common methods of labelling nucleic acid molecules are described next. Radioactive isotopes are often used to label nucleic acids, although they are more hazardous than non-radioactive labelling methods.

It is often difficult to make the link between theoretical and practical aspects of a subject, and an appreciation of the methods used in routine work with nucleic acids may be of help when the more detailed techniques of gene cloning and analysis are described. 1 Laboratory requirements One of the striking aspects of gene manipulation technology is that many of the procedures can be carried out with a fairly basic laboratory setup. Although applications such as large-scale DNA sequencing and production-scale biotechnology require major facilities and investment, it is still possible to do high-quality work within a ‘normal’ research laboratory.

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An introduction to genetic engineering by Dr Desmond S. T. Nicholl

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